Fine mapping of Brassica napus blackleg resistance gene Rlm1 through bulked segregant RNA sequencing
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چکیده
منابع مشابه
Fine Mapping of a Clubroot Resistance Gene in Chinese Cabbage Using SNP Markers Identified from Bulked Segregant RNA Sequencing
Clubroot, caused by Plasmodiophora brassicae, is an important disease of canola (Brassica napus) in western Canada and worldwide. In this study, a clubroot resistance gene (Rcr2) was identified and fine mapped in Chinese cabbage cv. "Jazz" using single-nucleotide polymorphisms (SNP) markers identified from bulked segregant RNA sequencing (BSR-Seq) and molecular markers were developed for use in...
متن کاملGene Mapping via Bulked Segregant RNA-Seq (BSR-Seq)
Bulked segregant analysis (BSA) is an efficient method to rapidly and efficiently map genes responsible for mutant phenotypes. BSA requires access to quantitative genetic markers that are polymorphic in the mapping population. We have developed a modification of BSA (BSR-Seq) that makes use of RNA-Seq reads to efficiently map genes even in populations for which no polymorphic markers have been ...
متن کاملIdentification of Genome-Wide Variants and Discovery of Variants Associated with Brassica rapa Clubroot Resistance Gene Rcr1 through Bulked Segregant RNA Sequencing.
Clubroot, caused by Plasmodiophora brassicae, is an important disease on Brassica species worldwide. A clubroot resistance gene, Rcr1, with efficacy against pathotype 3 of P. brassicae, was previously mapped to chromosome A03 of B. rapa in pak choy cultivar "Flower Nabana". In the current study, resistance to pathotypes 2, 5 and 6 was shown to be associated with Rcr1 region on chromosome A03. B...
متن کاملFine-Mapping the Branching Habit Trait in Cultivated Peanut by Combining Bulked Segregant Analysis and High-Throughput Sequencing
The growth habit of lateral shoots (also termed "branching habit") is an important descriptive and agronomic character of peanut. Yet, both the inheritance of branching habit and the genetic mechanism that controls it in this crop remain unclear. In addition, the low degree of polymorphism among cultivated peanut varieties hinders fine-mapping of this and other traits in non-homozygous genetic ...
متن کاملGenetic Dissection of Blackleg Resistance Loci in Rapeseed (Brassica napus L.)
Blackleg disease caused by the heterothallic ascomycete fungus Leptosphaeria maculans (Desm.) Ces. et de Not. (anamorph: Phoma lingam Tode ex Fr.), is the major disease of Brassica crops such as turnip rape (Brassica rapa L. syn. B. campestris; 2n = 2x = 20, genome AA), cabbage (B. oleracea L.; 2n = 2x = 18, genome CC), rapeseed (syn. canola or oilseed rape B. napus L.; 2n = 4x = 38, genome AAC...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2019
ISSN: 2045-2322
DOI: 10.1038/s41598-019-51191-z